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KMID : 1098420120200060461
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Korean Journal of Medicinal Crop Science
2012 Volume.20 No. 6 p.461 ~ p.465
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Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai
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Kim Hee-Young
Seong Eun-Soo
Lee Jae-Keun
Yoo Ji-Hye
Hwang In-Seong
Kim Myong-Jo
Lim Jung-Dae
Kim Na-Young
Yu Chang-Yeon
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Abstract
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This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of NH4NO3 and KNO3 at the ratio of 1650 (㎎/ℓ ) : 1900 (㎎/ℓ ) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with 170 ㎎/ℓ KH2PO4. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.
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KEYWORD
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Oplopanax elatus Nakai, Somatic Embryogenesis, Suspension Culture
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